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KMID : 0861020090240010099
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Korea Journal of Herbology
2009 Volume.24 No. 1 p.99 ~ p.110
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Effects of the Coptidis Rhizoma Extract on the Membranous Nephropathy induced by Cationic Bovine Serum Albumin in Mice
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Chae Eun-Young
Cho Chung-Sik
Kim Cheol-Jung
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Abstract
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Objectives: The current treatment regimens for patients with nephrotic syndrome due to membranous nephropathy(MN) are based on steroids or immunosuppressive therapy with the aim of reducing proteinuria and improving outcome. Although these treatments attenuate the deterioration of renal function in MN patients, it has been suggested that all are burdened by significant toxicity. Therefore, more specific and less toxic therapies are needed. This study was to evaluate the effects of Coptidis Rhizoma Extract(CRE) on the MN induced by cBSA in mice.
Methods: Mice were divided into 4 groups. One group named for ’Normal’ was injected with a saline solution not to be immunized. The rest groups were treated as follows; After mice were immunized with 0.2 mg of cBSA and Freund’s complete adjuvant one time every two weeks for 6 weeks, they received intra-peritoneal injection of 10 mg/kg of cBSA daily for 4 weeks. Also, they were divided into 3 groups. The first named for ’Control’ was not given CRE. The second for ’CRE-250’ was given oral administration of 250 mg/kg of CRE daily for 4 weeks. The third for ’CRE-500’ was given 500 mg/kg of CRE. All of mice were sacrificed 4 weeks after the first immunization. We measured a body weight and 24hrs proteinuria as well as serological analysis. The morphologic changes of renal glomeruli were also observed with a light microscope and an electron microscope.
Results: The levels of 24 hrs proteinuria, triglyceride, IgG, IL-6 were significantly decreased in both CRE groups. And the level of IgM was significantly decreased in CRE-250 group. In histological findings of kidney tissue, thickening of GBM and deposition of electron-density were consideraly decreased in both CRE groups.
Conclusions: The present study suggests that CRE is highly effective when treating mice with MN induced by cBSA. More clinical data and studies are to be done for efficient application.
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KEYWORD
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Coptis chinensis Extract(CRE), membranous nephropathy(MN), cationic bovine serum albumin(cBSA)
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